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Chapters
- 00:05Title
- 01:43Generation of Primary Mammospheres from Human Breast Cancer Cell Lines
- 04:54Generation of Primary Mammospheres from Human Breast Cancer Clinical Samples
- 07:04Mammosphere Forming Efficiency (%) Calculation
- 07:42Serial Passaging of Mammospheres for Assessment of Self-renewal
- 09:18Results: Estimation of Sphere Forming Efficiancy in MCF7 and MDA-231 Cells
- 10:04Conclusion
Floating mammosphere assays can investigate the subset of stem-like breast cancer cells that survive in suspension conditions and show enhanced tumorigenesis when implanted into mice. This protocol provides a convenient in vitro measure of sphere-forming ability, a proxy for in vivo tumorigenesis, while facilitating analysis of the stem-associated transcriptional landscape.
