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An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation
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An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation

An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation

DOI:
10.3791/52672-v

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08:48 min

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June 30, 2015

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, , ,
1Department of Medicine and New Jersey Medical School Cancer Center,Rutgers New Jersey Medical School

Chapters

  • 00:05Title
  • 01:36Clonogenic Assay Plate Preparation
  • 04:13Clonogenic Assay
  • 06:34Results: Pi3 Kinase Inhibitors Activate the Doormant Phenotype in Estrogen Sensitive Cells
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

We developed an in vitro model of dormancy in the bone marrow for estrogen-sensitive breast cancer cells. The goal of this protocol is to demonstrate use of the model for the study of the molecular and cellular biology of dormancy and for generation of hypotheses for subsequent testing in vivo.

Tags

Keyword Extraction: Breast Cancer DormancyIn Vitro Dormancy ModelEstrogen-sensitive Breast CancerBone MarrowClonogenic AssayFibronectinFGF-2Dormant CellsCell PhenotypeMolecular Mechanism StudiesHypothesis Generation

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