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1Section of Hepato-Pancreato-Biliary Surgery and Liver Transplantation,University of Groningen, University Medical Center Groningen, 2Surgical Research Laboratory, Department of Surgery,University of Groningen, University Medical Center Groningen, 3Center of Engineering in Medicine/Surgical Services,Massachusetts General Hospital, Harvard Medical School, and Shriners Burns Hospital, 4Division of Transplantation, Department of Surgery,Massachusetts General Hospital, Harvard Medical School
Chapters
- 00:05Title
- 01:50Priming of the Perfusion Device
- 04:43Procedure and Preparation of Donor Livers
- 07:10Normothermic Machine Perfusion
- 10:06Results: Donor Livers are Well Preserved by Ex Situ Normothermic Machine Perfusion
- 11:46Conclusion
Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
