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Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
 

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Article DOI: 10.3791/52691-v • 07:36 min • May 1st, 2015
May 1st, 2015 •
1,2, 1,3, 1, 1,2,3, 1,2, 1,2,3
1Murdoch Childrens Research Institute, The Royal Children’s Hospital, 2Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, 3Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton

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Summary
Automatic Translation

The goal of this protocol is to isolate lymphatic endothelial cells lining human lymphatic malformation cyst-like vessels and foreskins using fluorescence-activated cell sorting (FACS). Subsequent cell culturing and expansion of these cells permits a new level of experimental sophistication for genetic, proteomic, functional and cell differentiation studies.

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Keywords: Lymphatic Endothelial Cells Lymphatic System Disorders Primary Lymphedema Lymphatic Malformations Lymphatic Tumors Fluorescence-activated Cell Sorting CD34 CD31 VEGFR-3 Podoplanin Cell Isolation Cell Culture
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