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組織学的分析のために筋肉凍結切片の調製でのやっていいことといけないことを行います
JoVE Journal
Medicine
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JoVE Journal Medicine
Do’s and Don’ts in the Preparation of Muscle Cryosections for Histological Analysis
DOI:

10:27 min

May 15, 2015

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Chapters

  • 00:05Title
  • 02:02Harvesting and Freezing Tissue
  • 04:12Embedding Tissues in OCT
  • 06:12Preparing Sections and Identifying Mid-belly Region Muscles
  • 07:44Notes on Staining and Mounting
  • 08:53Results
  • 09:37Conclusion

Summary

Automatic Translation

Here we demonstrate the most efficient methods for freezing, embedding, cryosectioning, and staining of muscle biopsies to avoid freezing artifacts.

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