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Chapters
- 00:05Title
- 02:35Kidney Flush
- 04:25Isolation of the Rat Glomeruli
- 05:35Single-channel Patch-clamp Electrophysiology
- 06:30Ratiometric Confocal Fluorescence Measurements of Intracellular Calcium Concentration in the Podocytes
- 08:08Image Analysis for the Calcium Measurements
- 09:46Results: Recording of Single Ion Channels Activity in the Podocytes of Freshly Isolated Glomeruli and Real-time Detection of Calcium Concentration Changes
- 11:16Conclusion
Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.
