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Co-immunoprecipitation av musen Mx1 Protein med influensa A virus nucleoprotein
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Immunology and Infection
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JoVE Journal Immunology and Infection
Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein

Co-immunoprecipitation av musen Mx1 Protein med influensa A virus nucleoprotein

DOI:
10.3791/52871-v

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09:39 min

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April 21, 2015

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, ,
1Inflammation Research Center,VIB, 2Department of Biomedical Molecular Biology,Ghent University

Chapters

  • 00:05Title
  • 01:28Calcium-phosphate Transfection of HEK293T Cells
  • 03:05Preparation of Cell Lysates for Co-immunoprecipitation
  • 04:09Preparation of Immune Complexes and Protein G Beads
  • 05:38Immunoprecipitation of the Immune Complexes by Protein G Beads and their Elution
  • 08:00Results: Mx1 Interacts with Influenza A Virus Nucleoprotein
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

This co-immunoprecipitation protocol allows to study the interaction between the influenza A virus nucleoprotein and the antiviral Mx1 protein in human cells. The protocol emphasizes the importance of N-ethylmaleimide for successful co-immunoprecipitation of Mx1 and influenza A virus nucleoprotein.

Tags

Keywords: Co-immunoprecipitationMx1 ProteinInfluenza A Virus NucleoproteinProtein InteractionAntiviralN-ethylmaleimideProtein ComplexWestern BlottingProtein G BeadsFalse PositiveControl Experiments

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