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अलगाव और माउस महाधमनी endothelial कोशिकाओं के प्राथमिक संस्कृति
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
DOI:

08:20 min

December 19, 2016

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Chapters

  • 00:05Title
  • 00:54Preparing Aortic Segments
  • 03:14Seeding Aortic Segments on a Matrix
  • 04:48Passaging the Endothelial Cells
  • 06:41Aorta-derived Endothelial Cell Characterization
  • 07:48Conclusion

Summary

Automatic Translation

The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.

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