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एक<em> इन विट्रो</em> एचआईवी सह-संक्रमण के संदर्भ में मलेरिया के लिए इम्यून प्रतिक्रियाओं को मापने के लिए मॉडल
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
An In Vitro Model for Measuring Immune Responses to Malaria in the Context of HIV Co-infection
DOI:

08:14 min

October 06, 2015

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Chapters

  • 00:05Title
  • 00:57Parasite Synchronization and Preparation for Co-culture
  • 02:27Human Peripheral Blood Mononuclear Cell (PBMC) Isolation
  • 03:42Malaria/HIV Co-infection Culture and Detection of Malaria-specific Immune Responses
  • 04:52Cell-specific Cytokine Production by PBMC Co-culture with P. falciparum Infected RBC
  • 06:57Results: Representative IFNγ Production by Natural Killer T Cells
  • 07:38Conclusion

Summary

Automatic Translation

Human co-infection is difficult to replicate in vitro. However, human malaria parasites can readily be cultured in vitro, as can freshly isolated human peripheral blood mononuclear cells naturally infected with HIV. This provides an excellent model for studying early immune responses to malaria parasites in the context of HIV co-infection.

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