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TRAP-rc, Translating Ribosome Affinity Purification da popolazioni di cellule rare<em> Drosophila</em> Embrioni
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Biology
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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos
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TRAP-rc, Translating Ribosome Affinity Purification da popolazioni di cellule rare<em> Drosophila</em> Embrioni

DOI:
10.3791/52985-v

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10:26 min

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September 10, 2015

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, , , ,
1University of Clermont-Ferrand, 2Memorial Sloan Kettering Cancer Center

Chapters

  • 00:05Title
  • 02:09Amplification of TRAP Line
  • 04:29Lysate Preparation
  • 06:10Pre-absorption
  • 07:03Immunopurification
  • 08:35Results: Quality and Specificity of TRAP-Isolated RNA from Slouch-GAL4>UASRpL10A-EGFP Embryos
  • 09:56Conclusion

Summary

Automatic Translation

Automatic Translation

Translating Ribosome Affinity Purification (TRAP) is able to capture cell-type-specific translation of mRNA. Here we report the first TRAP protocol dedicated to isolation of mRNA in rare cell populations of Drosophila embryos.

Tags

Keywords: TRAPTranslating Ribosome Affinity PurificationRare Cell PopulationsDrosophila EmbryosMRNA TranslationCell-type-specificTranslatome AnalysisPolysomesTagged Ribosomal SubunitDevelopmental ProcessesDisease ProgressionCell-specific Gene ExpressionMicroarrayRNA-seqGal4/UAS SystemMuscle Cells

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