Journal
/
Biology
/
Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

DOI:
10.3791/53059-v

•

10:00 min

•

October 12, 2015

•

, , , , ,
1Department of Physiology,Medical College of Wisconsin

Chapters

  • 00:05Title
  • 02:03Sensor Calibration
  • 04:38Animal Surgeries
  • 06:09Data Acquisition Ex Vivo
  • 07:23Results: Microelectrode Biosensor Detection of Analytes in Whole Kidneys
  • 08:57Conclusion

Summary

Automatic Translation

Automatic Translation

Enzymatic microelectrode biosensors enable real-time measurements of extracellular cell signaling in biologically-relevant concentrations. The following protocols extend the applications of biosensors to the ex vivo and in vivo detection of ATP and H2O2 in the kidney.

Tags

Enzymatic BiosensorsATPH2O2KidneyReal-time DetectionAmperometryGlycerol KinaseGlycerol-3-phosphate OxidasePlatinum/iridium ElectrodeGold ElectrodeCatalaseApyraseCalibrationEx VivoIn Vivo

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53059?article_type=v

The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF
check_url/53059?article_type=v

Dissection of the Adult Zebrafish Kidney
check_url/53059?article_type=v

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
check_url/53059?article_type=v

The Use of Chemostats in Microbial Systems Biology
check_url/53059?article_type=v

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
check_url/53059?article_type=v

Measuring Lactase Enzymatic Activity in the Teaching Lab
check_url/53059?article_type=v

The Mouse Isolated Perfused Kidney Technique
check_url/53059?article_type=v

Techniques to Induce and Quantify Cellular Senescence
check_url/53059?article_type=v

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
check_url/53059?article_type=v

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Read Article