/
/
Brug af Konfokal Analyse af<em> Xenopus laevis</em> At Undersøg Modulatorer af Wnt og Shh Morfogen Gradienter
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Biomedical Science,The Bateson Centre, University of Sheffield, 2Institute of Genetic Medicine,Newcastle University, 3Department of Cardiovascular Science,The Bateson Centre, University of Sheffield, 4School of Biochemistry,University of Bristol, 5Biology Department,University of York
Chapters
- 00:05Title
- 01:09Microinjecting Xenopus Embryos
- 02:10Excising Animal Cap Explants
- 04:17Animal Explant Imaging
- 06:04Results: Diffusion Analysis of Fluorescently Tagged Ligands in Xenopus Animal Cap Explants
- 07:38Conclusion
Manuskriptet her giver et simpelt sæt af metoder til analyse sekretion og udbredelse af fluorescens mærkede ligander i Xenopus. Dette giver en kontekst for at afprøve, om andre proteiner for at modificere ligand distribution og tillade eksperimenter, der kan give indsigt i mekanismer, der regulerer morfogen gradienter.
