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Chapters
- 00:05Title
- 00:42Mouse Mammary Gland Dissection
- 01:56Mammary Tissue Fixation and Embedding
- 03:23Frozen Tissue Sectioning
- 05:20Indirect Immunofluorescence
- 08:47Results: Mapping Proteins Involved with Lactation
- 09:53Conclusion
The indirect immunofluorescence protocol described in this article allows the detection and the localization of proteins in the mouse mammary gland. A complete method is given to prepare mammary gland samples, to perform immunohistochemistry, to image the tissue sections by fluorescence microscopy, and to reconstruct images.
