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Monitoring Endoplasmic Reticulum Calcium Homeostasis Using a Gaussia Luciferase SERCaMP
 

Monitoring Endoplasmic Reticulum Calcium Homeostasis Using a Gaussia Luciferase SERCaMP

Article DOI: 10.3791/53199-v • 08:41 min • September 6th, 2015
September 6th, 2015 •
*1, *1, 1, 1, 1
1National Institute on Drug Abuse, National Institutes of Health
* These authors contributed equally

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Summary
Automatic Translation

Endoplasmic reticulum calcium homeostasis is disrupted in diverse pathologies. A secreted ER calcium monitoring protein (SERCaMP) reporter can be used to detect disruptions in the ER calcium store. This protocol describes the use of a Gaussia luciferase SERCaMP to examine ER calcium homeostasis in vitro and in vivo.

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Keywords: Endoplasmic Reticulum ER Calcium Homeostasis Gaussia Luciferase SERCaMP Unfolded Protein Response ER Stress In Vivo Calcium-dependent Fluorescent Dyes Protein Folding Protein Modification Protein Trafficking Disease Pathogenesis Longitudinal Monitoring
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