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Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
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Immunology and Infection
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JoVE Journal Immunology and Infection
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

DOI:
10.3791/53214-v

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10:22 min

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December 04, 2015

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, , , ,
1Department of Molecular Genetics and Microbiology,Duke University School of Medicine, 2Center for Genomic and Computational Biology,Duke University School of Medicine, 3Department of Cell Biology,Duke University School of Medicine

Chapters

  • 00:05Title
  • 00:50Bio-tagging and Elution of miRNA-mRNA Fusion Products
  • 03:02Polysome Separation to Determine the Ribosomal Occupancy of P. falciparum
  • 08:34Results: Erythrocyte mRNA Enrichment and Parasite Polysomes
  • 09:32Conclusion

Summary

Automatic Translation

Automatic Translation

Human microRNAs translocate from host erythrocytes to Plasmodium falciparum parasites. Here, the techniques used to transfect synthetic microRNAs into host erythrocytes and isolate all RNAs from P. falciparum are described. In addition, this paper will detail a method of polysome isolation in P. falciparum to determine the ribosomal occupancy and translational potential of parasite transcripts.

Tags

Keywords: MicroRNARibosomal OccupancyPlasmodium FalciparumSickle CellErythrocytesGene RegulationTranslationPolysome Gradient

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