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Chapters
- 00:05Title
- 01:38Basic Setup
- 02:50SVZ Tissue Dissociation
- 04:19Immunofluorescent Staining and Cell Sorting
- 06:21Microscope Setup and Image Acquisition
- 07:57Results: Live Imaging of the Cell Cycle Phases at the Level of a Single Cell
- 08:53Conclusion
We report a Fluorescent Activated Cell Sorting (FACS)-based method to isolate neural stem cells (NSCs) and their progeny from the subventricular zone (SVZ) of the adult mouse brain. Applied to Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) transgenic mice, it allows the study of cell cycle progression by live imaging.
