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Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma
 

Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma

Article DOI: 10.3791/53287-v • 09:17 min • January 15th, 2016
January 15th, 2016 •
1, 2, 2, 2,3
1Department of Neurological Surgery, University of California San Francisco, 2Department of Neurological Surgery, Northwestern University Feinberg School of Medicine, 3Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine

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Summary
Automatic Translation

GL261 glioma cells provide a useful immunocompetent animal model of glioblastoma. The goals of this protocol are to demonstrate proper techniques for monitoring intracranial tumor growth using in vivo bioluminescence imaging, and to verify the utility of luciferase-modified GL261 cells for studying tumor immunology and immunotherapeutic approaches for treating glioblastoma.

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Keywords: Bioluminescence Imaging Immunocompetent Animal Model Glioblastoma GL261 Murine Tumor Cells Luciferase Modification Tumor Cell Proliferation In Vivo Monitoring Tumor Growth Immunotherapy In Vitro Proliferation Assay MTS Reagent Absorbance Measurement
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