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Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

DOI:
10.3791/53376-v

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07:54 min

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October 15, 2015

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, , ,
1Department of Bio and Brain Engineering,KAIST, 2Information and Electrical Research Institute,KAIST

Chapters

  • 00:05Title
  • 00:45Preparation of Phospholipid Monolayer at a Flat Air-water Interface
  • 02:32Preparation of Phospholipid Monolayer at the Curved Surface of a Droplet
  • 04:00Imaging Fluorescence Recovery after Droplet Merging
  • 05:17Results: Diffusion Coefficient of DOPC and DPPC Monolayer as a Function of Surface Pressure
  • 06:39Conclusion

Summary

Automatic Translation

Automatic Translation

We present a new technique to measure the lateral diffusion of a surface active species at the fluid-fluid interface by merging a droplet monolayer onto a flat monolayer.

Tags

Keywords: Fluorescence RecoveryMerging DropletTwo-dimensional DiffusionPhospholipid MonolayerFRAMFRAPSurfactant MonolayerFluid-fluid InterfaceLateral DiffusivityCoalescenceFluorescence MicroscopeDye MoleculesDiffusion Area

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