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Fysisk Isolering af endosporer fra miljøprøver ved målrettet Lysis af vegetative celler
JoVE Journal
Environment
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JoVE Journal Environment
Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
DOI:

09:38 min

January 21, 2016

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Chapters

  • 00:05Title
  • 01:01Separation of Biomass from Sediment
  • 02:36Collection of Biomass on Filter Membrane
  • 03:31Lysis of Vegetative Cells
  • 05:44Dnase Treatment
  • 07:02Results: Endospore Isolation from Lysed Vegetative Cells
  • 08:26Conclusion

Summary

Automatic Translation

A method to single out bacterial endospores from complex microbial communities was developed to perform tailored culture or molecular studies of this group of bacteria.

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