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Immunology and Infection

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JoVE Journal Immunology and Infection
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
 

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Article DOI: 10.3791/53424-v • 07:45 min • November 28th, 2015
November 28th, 2015 •
1, 1, 1
1Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam

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Summary
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Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.

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Keywords: Macrophage Polarization M1 Macrophages M2 Macrophages Metabolic Reprogramming Glycolysis Oxidative Phosphorylation Extracellular Flux Analysis Oxygen Consumption Rate Extracellular Acidification Rate Mitochondrial Activity Spare Respiratory Capacity
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