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En hurtigt Air-tør droppe kromosom fremstillingsmetode Egnet til FISH i planter
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JoVE Journal Biology
A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

En hurtigt Air-tør droppe kromosom fremstillingsmetode Egnet til FISH i planter

DOI:
10.3791/53470-v

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09:08 min

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December 16, 2015

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, ,
1Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 2School of Biological and Chemical Sciences,Queen Mary University of London

Chapters

  • 00:05Title
  • 01:00Chromosome Preparation
  • 05:25Fluorescent In Situ Hybridization (FISH)
  • 07:11Results: FISH Performed on Mitotic Metaphase Chromosome Spreads of Hordeum vulgare, H. Bulbosum, and Secale cereale Prepared by the Aid-dry Dropping Method
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol is described for the preparation of high-quality mitotic plant chromosome spreads by a fast air-dry dropping method suitable for the FISH detection of single and high copy DNA probes.

Tags

Chromosome Spread PreparationFISHAir-dry Drop MethodPlant CytogeneticsHordeumSecale

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