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Fecal Glukokortikoid analyse: Ikke-invasiv Adrenal Monitoring i Equids
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JoVE Journal Biology
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

Fecal Glukokortikoid analyse: Ikke-invasiv Adrenal Monitoring i Equids

DOI:
10.3791/53479-v

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08:02 min

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April 25, 2016

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, ,
1School of Animal Rural and Environmental Science,Nottingham Trent University, 2Chester Zoo, Upton-by-Chester

Chapters

  • 00:05Title
  • 00:43Fecal Hormone Extraction
  • 02:08Hormone Analysis
  • 03:48Enzyme Linked Immunoassay (EIA): Validation for the Study of Species and Sex
  • 05:41Results: Biochemical (Parallelism) and Biological Validation of a EIA for male and Female Horses
  • 06:32Conclusion

Summary

Automatic Translation

Automatic Translation

Adrenal activity can be assessed in the equine species by analysis of feces for corticosterone metabolites. The method offers a non-invasive option to assess long term patterns in both domestic and free ranging horses. This protocol describes the enzyme linked immunoassay involved and the associated biochemical validation.

Tags

Keywords: Fecal Glucocorticoid AnalysisNon-invasive Adrenal MonitoringEquidsWelfare AssessmentDomestic HorsesFree-ranging HorsesMethanol ExtractionCorticosteroneEnzyme-linked ImmunoassayEIA

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