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Hele Mount Dissektion og Immunofluorescens af Voksen Mouse Cochlea
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
DOI:

12:02 min

January 01, 2016

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Chapters

  • 00:05Title
  • 00:59Preparation of Temporal Bones
  • 02:14Whole Mount Dissection of the Cochlea
  • 05:41Immunostaining with Fluorescently Tagged Antibodies
  • 07:30Mounting Stained Cochlear Turns
  • 08:59Results: Examples of Optimal and Suboptimal Dissection and Immunostaining
  • 10:54Conclusion

Summary

Automatic Translation

Vi præsenterer en metode til at isolere voksne organ Corti som tre intakte cochlear sving (apex, midten og bunden). Vi viser også en procedure for immunfarvning med fluorescens mærkede antistoffer. Tilsammen gør disse teknikker gør det muligt studiet af hårceller, støtteceller, og andre celletyper fundet i cochlea.

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