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从鼠耳和尾组织生成主成纤维细胞培养
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Developmental Biology
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JoVE Journal Developmental Biology
Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
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从鼠耳和尾组织生成主成纤维细胞培养

DOI:
10.3791/53565-v

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06:35 min

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January 10, 2016

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,
1Immunology Program, Department of Microbiology,Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering,National University of Singapore

Chapters

  • 00:05Title
  • 00:42Extraction of Fibroblasts from Ear and Tail Tissues
  • 03:20Cell Culture and Sub-Culturing of Fibroblasts
  • 05:05Results: Fibroblast Culture Hardiness and Purity
  • 06:03Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述用于产生从耳朵和小鼠的尾部的成纤维细胞初级一个简单而快速的实验程序。该过程不需要特殊的动物训练和可用于成纤维细胞培养的产生从存储在RT至10天耳朵。

Tags

Keywords: Primary FibroblastMouse EarMouse TailCell IsolationCollagenase DigestionCell StrainerCell SuspensionCell Washing

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