/
/
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:03Low-magnification Map Collection
- 03:22Tilt-series Collection
- 05:30High-throughput Automated Tilt-series Processing and Reconstruction Using Tomoauto
- 09:08Results: Tomogram and Sub-tomogram Views of S. flexneri
- 10:31Conclusion
We present a protocol on how to utilize high-throughput cryo-electron tomography to determine high resolution in situ structures of molecular machines. The protocol permits large amounts of data to be processed, avoids common bottlenecks and reduces resource downtime, allowing the user to focus on important biological questions.
Tags
TomoautoHigh-throughputAutomated Cryo-electron TomographyData HandlingNew Generation DetectorsAutomated Tilt-series Collection SoftwareSoftware PackagesPipelineTomogram AnalysisCryo-ETElectron Microscope3D ReconstructionBacteriaShigella Flexneri MinicellsElectron Microscopy GridsLow-magnification MapsFluorescent Screen
