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Chapters
- 00:05Title
- 00:46IR700 Conjugation to Monoclonal Antibodies (mAb)
- 01:42Mixed 3D Cell Culture In Vitro Near Infrared Photoimmunotherapy (NIR-PIT)
- 04:11Results: Target cell Elimination in 3D Cell Spheroids
- 04:49Conclusion
Eliminating specific cells without damaging other cells is extremely difficult, especially in established tissue, yet there is an urgent need for a cell elimination method in the tissue engineering field. Here, we present a method for specific cell elimination from a mixed 3D cell culture using near infrared photoimmunotherapy (NIR-PIT).
Tags
Selective Cell EliminationMixed 3D CultureNear Infrared PhotoimmunotherapyRegenerative MedicineTarget MoleculesSpecific LesionsSide EffectsIR700 ConjugationMonoclonal AntibodiesIncubationAluminum FoilPD10 ColumnPBS ElutionSpectrophotometerProtein Concentration3D Cell CultureSterile WaterHanging Drop Plate
