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Modellering Kjemoterapi Resistant Leukemi<em> In Vitro</em
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JoVE Journal Medicine
Modeling Chemotherapy Resistant Leukemia In Vitro

Modellering Kjemoterapi Resistant Leukemi<em> In Vitro</em

DOI:
10.3791/53645-v

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08:41 min

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February 09, 2016

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, , , , , , ,
1Alexander B. Osborn Hematopoietic Malignancy and Transplantation Program of the Mary Babb Randolph Cancer Center, Robert C. Byrd Health Sciences Center,West Virginia University School of Medicine, 2Department of Neurobiology and Anatomy, Robert C. Byrd Health Sciences Center,West Virginia University School of Medicine, 3Department of Microbiology, Immunology and Cell Biology, Robert C. Byrd Health Sciences Center,West Virginia University School of Medicine

Chapters

  • 00:05Title
  • 00:37Co-culture Maintenance
  • 02:24Gel Type 10 Cross-linked Dextran (G10) Bead Column Preparation
  • 04:08Subpopulation Separation
  • 06:06G10 Column Leukemic Cell Collection
  • 07:26Results: PD Leukemic Cells Exhibit an Increased Resistance to Chemotherapy Exposure
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

The current report summarizes a protocol that can be utilized to model the influence of the bone marrow microenvironment niche on leukemic cells with emphasis placed on enrichment of the most chemoresistant subpopulation.

Tags

ModelingChemotherapy Resistant LeukemiaIn VitroAcute Lymphoblastic LeukemiaRefractory ALLBone Marrow MicroenvironmentChemotherapeutic ResistanceDrug AdvocacyAnimal ModelsLeukemic CellsTumor-specific Culture MediumBone Marrow Stromal CellsOsteoblastsCo-cultureAdherent Cell LayerLeukemic Cell Culture MediumFloating Leukemic Cells15 Milliliter Conical TubeSubpopulations

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