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Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy
 
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Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy

Article DOI: 10.3791/53657-v • 09:29 min • May 4th, 2016
May 4th, 2016 •
1, 1, 2, 1,2,3
1Department of Cancer Biology, Thomas Jefferson University, SKCC, 2Department of Medical Oncology, Thomas Jefferson University, SKCC, 3Kazan Federal University

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Herein, we describe in detail a time-lapse video microscopy approach to measuring the temporal recruitment of EYFP-Parkin during the selective removal of damaged mitochondria. This dynamic process of EYFP-Parkin-dependent removal of damaged mitochondria can be used as an indicator of cellular health under different experimental conditions.

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Time-Lapse Video Microscopy EYFP-Parkin Aggregation Cellular Mitophagy Molecular Metabolism Labeled Proteins Mitochondrial Life Cycle Primary Cell Cultures Embryology Studies Predicting Priority Immortalized Mouse Embryonic Fibroblasts DMEM Medium Trypsin EDTA Cell Suspension
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