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性别差异在小鼠海马星形胶质细胞<em>在体外</em>缺血
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Neuroscience
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JoVE Journal Neuroscience
Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia
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性别差异在小鼠海马星形胶质细胞<em>在体外</em>缺血

DOI:
10.3791/53695-v

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08:32 min

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October 25, 2016

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, , , , ,
1Waisman Center,University of Wisconsin, 2Department of Neurological Surgery,University of Wisconsin, 3Department of Pediatrics,University of Wisconsin

Chapters

  • 00:05Title
  • 00:42Hippocampal Dissection and Plating
  • 03:32Astrocyte Culture
  • 05:34Oxygen and Glucose Deprivation and Reoxygenation (OGD/REOX)
  • 06:21Results: Increased Protein Expression of HIF1α and Astrocytic Markers Following OGD/REOX
  • 07:46Conclusion

Summary

Automatic Translation

Automatic Translation

星形胶质细胞是中枢神经系统（CNS）最重要的关键球员之一。在这里，我们报告，以研究男性和女性新生儿幼崽的星形胶质细胞功能的体外缺血后背后的机制性控海马星形胶质细胞培养协议的实用方法。

Tags

Sex DifferencesMouse Hippocampal AstrocytesIn-vitro IschemiaEnriched CulturesOxygen And Glucose DeprivationReoxygenationHypoxic IschemiaMechanistic QuestionsTranslational QuestionsDeveloping Male And Female BrainsBrain DissectionNewborn Mouse PupPosterior To Anterior Midline IncisionCranium RemovalBrainstem RemovalDissecting DishHemispheres SeparationCerebral Hemispheres PeelingHippocampus RemovalMeninges And Fibroblasts Cleaning

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