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在水中水合蛋白原位表征SALVI和TOF-SIMS

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Chemistry

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JoVE Journal Chemistry

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

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在水中水合蛋白原位表征SALVI和TOF-SIMS

DOI:

10.3791/53708-v

•

09:48 min

•

February 15, 2016

•

Jiachao Yu, Yufan Zhou*², Xin Hua*¹, Zihua Zhu, Xiao-Ying Yu

¹Fundamental & Computational Sciences Directorate,Pacific Northwest National Laboratory, ²Wiley Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory

Chapters

00:05Title
01:03Cleaning and Sterilization of the SALVI Microchannel
02:05Immobilization of the Protein Film in SALVI
02:50Install SALVI into the ToF-SIMS Loadlock Chamber
04:05ToF-SIMS Data Acquisition
07:35Results: Dynamic Liquid Surface and Liquid-solid Interface Analysis
09:02Conclusion

Summary

Automatic Translation

这项工作提出了液体处理和样品引入用于原位时间飞行二次离子质谱法的蛋白质的生物分子的水溶液中分析一个协议到一个微通道。

Tags

In Situ Characterization Hydrated Proteins Water SALVI ToF-SIMS Biomolecules Biointerface Solid-liquid Interface Cancer Cells Drug Interactions Biofilms Fibronectin Silicon Wafer Silicon Nitride Membrane

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