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Nye beregninger for å karakter Embryonic Forlengelse av nematode<em> Caenorhabditis elegans</em
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JoVE Journal Neuroscience
Novel Metrics to Characterize Embryonic Elongation of the Nematode Caenorhabditis elegans

Nye beregninger for å karakter Embryonic Forlengelse av nematode<em> Caenorhabditis elegans</em

DOI:
10.3791/53712-v

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10:46 min

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March 28, 2016

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, ,
1Department of Chemistry, Pharmaqam,Université du Québec à Montréal

Chapters

  • 00:05Title
  • 00:52Characterizing Early Elongation Using 4D (Time Lapse Z-stack) DIC Imaging
  • 03:47Characterizing Late Elongation Using Flow Cytometry
  • 08:23Results: 4D-DIC versus Flow Cytometry for Sizing Nematode Larvae
  • 09:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here we detail protocols specifically designed to monitor morphogenic defects that occur during early and late phases of embryonic elongation of the nematode Caenorhabditis elegans. Ultimately, these protocols are designed to identify genes that regulate these phases and to characterize their differential requirements along the antero-posterior axis of the embryo.

Tags

Novel MetricsEmbryonic ElongationNematodeCaenorhabditis ElegansDevelopmental DefectsDevelopmental GeneticsGenes ControlMorphogenic ProcessesDIC LensesPrismVideo Capture SystemTime-lapse MicroscopyZ StacksPre-morphogenesis StagesOil DropPowerful ObjectiveZ Stack ParametersTime-lapse Imaging

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