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Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment
 

Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment

Article DOI: 10.3791/53741-v • 11:19 min • February 3rd, 2016
February 3rd, 2016 •
*1, *1, 1,2, 1,3
1Department of Anatomy, University of California, 2Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine
* These authors contributed equally

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Summary
Automatic Translation

We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.

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Keywords: Ex Vivo Live Imaging Lung Metastasis Tumor Cell Stroma Interactions Metastasis Establishment Cell Types Microenvironment Targeted Therapeutics Lung Imaging Cell Death Mouse Trachea Agarose
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