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Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis
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Neuroscience
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JoVE Journal Neuroscience
Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis

DOI:
10.3791/53743-v

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14:28 min

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March 16, 2016

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, ,
1Curriculum in Neurobiology,The University of North Carolina at Chapel Hill, 2Department of Cell Biology and Physiology,The University of North Carolina at Chapel Hill

Chapters

  • 00:05Title
  • 00:48SNARE Complex Formation Assay
  • 03:12Imaging Exocytic Events via TIRF Microscopy
  • 08:08Differential Interference Contrast Timelapse Microscopy of Axon Branching
  • 10:10Toxin Manipulations and Fixed Cell Immunofluorescence
  • 11:13Results: SNARE-mediated Exocytosis in Netrin-dependent Axon Branching
  • 12:58Conclusion

Summary

Automatic Translation

Automatic Translation

Light microscopy techniques coupled with biochemical assays elucidate the involvement of SNARE-mediated exocytosis in netrin-dependent axon branching. This combination of techniques permits identification of molecular mechanisms controlling axon branching and cell shape change.

Tags

Combined MethodologiesPlasma Membrane DeliveryAxon BranchingNeuronal MorphogenesisQuantifiable ObservationsTemporal And Spatial OccurrenceExocytic Vesicle FusionNeuronal DevelopmentLive Cell ImagingHigh Spatial ResolutionHigh Temporal ResolutionBiochemical ApproachesPopulation-based InformationCulturing And Simulating Cortical NeuronsNetrin-1ControlHomogenization BufferProtein ConcentrationsTriton X-100Triturate MixtureSolubilize ProteinsCentrifuge

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