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Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration
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Developmental Biology
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JoVE Journal Developmental Biology
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration

Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration

DOI:
10.3791/53750-v

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07:44 min

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March 18, 2016

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, ,
1Aab Cardiovascular Research Institute,University of Rochester School of Medicine and Dentistry, 2Department of Medicine,University of Rochester School of Medicine and Dentistry, 3Department of Pharmacology and Physiology,University of Rochester School of Medicine and Dentistry

Chapters

  • 00:05Title
  • 00:49Epicardial Outgrowth Culture and Genetic Ablation
  • 03:31Epicardial Labeling and Ex Vivo Migration Culture
  • 05:17Results: Representative Epicardial Outgrowth Purity
  • 06:23Representative Ex Vivo Migration Assay
  • 06:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.

Tags

Keywords: Epicardial OutgrowthEpicardial-derived Cell MigrationEx Vivo AssessmentPrimary Epicardial Cell CultureEpithelial-to-mesenchymal TransitionCardiac Regenerative MedicineCollagen-coated Chamber SlidesMouse EmbryoExplant MediumMesothelial CellsMesenchymal Cell ContaminationTrizol

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