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1Greenebaum Cancer Center,University of Maryland School of Medicine, 2Pharmaceutical Sciences,University of Maryland School of Pharmacy, 3Baltimore VA Medical Center, 4Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences,Kanazawa University, 5Obstetrics, Gynecology and Reproductive Science,University of Pittsburgh, 6Magee Women’s Research Institute, 7Obstetrics, Gynecology, Perinatal Research Branch (NICHD),Wayne State University School of Medicine, 8Medicine,University of Maryland School of Medicine, 9Pathology,University of Maryland School of Medicine, 10Pharmacology,University of Maryland School of Medicine, 11Experimental Therapeutics,University of Maryland School of Medicine
Chapters
- 00:05Title
- 01:04In Silico Prediction of Aternative First Exons of Bcrp1 Using BLAST Analysis of Mouse EST Database
- 04:30Design of Reporter Constructs for Bcrp1 E1U, E1A, E1B, and E1C Promoters
- 07:55Reporter Assay Methods
- 09:03Results: Evaluation of Bcrp1 Alternative Promoter Function
- 10:07Conclusion
Med mus ABC-transport Bcrp1 (ABCG2) som ett exempel, in silico-protokoll presenteras för att upptäcka alternativa promotor användning i gener som uttrycks i mus vävnader, och att utvärdera funktionaliteten av de alternativa promotorer identifieras med hjälp av reporteranalyser.
