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High-throughput CRISPR Vector Konstruktion og karakterisering af DNA Ændringer af Generation of Tomato Behårede Roots
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Biology
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JoVE Journal Biology
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

High-throughput CRISPR Vector Konstruktion og karakterisering af DNA Ændringer af Generation of Tomato Behårede Roots

DOI:
10.3791/53843-v

•

12:59 min

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April 30, 2016

•

,
1Boyce Thompson Institute for Plant Research, 2Section of Plant Pathology & Plant-Microbe Biology, School of Integrative Plant Science,Cornell University

Chapters

  • 00:05Title
  • 00:36CRISPR Vector Construction
  • 06:36Hairy Root Transformation
  • 10:59Results: Hairy Root Cultures and Detection of DNA Modification
  • 12:17Conclusion

Summary

Automatic Translation

Automatic Translation

Anvendelse af DNA samling, kan flere CRISPR vektorer konstrueres parallelt i en enkelt kloning reaktion, hvilket gør konstruktionen af ​​et stort antal CRISPR vektorer en enkel opgave. Tomat hårrødder er en glimrende modelsystem til at validere CRISPR vektorer og generere mutant materialer.

Tags

CRISPRVector ConstructionDNA ModificationsTomato Hairy RootsFunctional GenomicsCloning ProcedureGuide RNAKnockout MutantsPCR AmplificationHigh-ThroughputDNA Assembly

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