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Chapters
- 00:05Title
- 01:34Preparation of Polysome Lysate and Sucrose Gradient Fractionation
- 03:40Atomic Force Microscopy Sample Preparation
- 05:24AFM Imaging
- 06:40Results: AFM Reveals Ribosome/Polysome Details
- 07:39Conclusion
While ribosome structure has been extensively characterized, the organization of polysomes is still understudied. To overcome this lack of knowledge, we present here a detailed preparation protocol for accurate imaging of mammalian polysomes by atomic force microscopy (AFM) in air and liquid.
