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Fluoreszenz Zeitraffer-Bildgebung des kompletten<em> S. venezuelae</em> Life Cycle Mikrofluidikvorrichtung Verwendung
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Chapters
- 00:05Title
- 01:04Isolation of Fresh S. venezuelae Spores and Preparation of Spent Culture Medium
- 03:04Preparation of the Microfluidic Device
- 04:59Setup of the Microscope and the Time-lapse Protocol
- 09:16Results: Fluorescence Time-lapse Imaging of the Complete Life Cycle Using a Microfluidic Device
- 10:45Conclusion
Streptomyces are characterized by a complex life cycle that has been experimentally challenging to study by cell biological means. Here we present a protocol to perform fluorescence time-lapse microscopy of the complete life cycle by growing Streptomyces venezuelae in a microfluidic device.
Tags
Keywords: Fluorescence Time-lapse ImagingStreptomyces VenezuelaeLife CycleMicrofluidic DeviceCell DifferentiationSporulationPolarized GrowthProtein LocalizationMulticenter MyceliumSporesSupplemented Growth MediumLiquid CulturePhase Contrast MicroscopyCentrifugationSpore SuspensionSpent MediumSyringe FilterMicrofluidic Plate
