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高效的核酸提取和16S rRNA基因序列的细菌群落特征

JoVE Journal
Biology

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JoVE Journal Biology

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

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高效的核酸提取和16S rRNA基因序列的细菌群落特征

DOI:

10.3791/53939-v

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12:37 min

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April 14, 2016

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Melis N. Anahtar, Brittany A. Bowman, Douglas S. Kwon

¹Ragon Institute of MGH, MIT, and Harvard,Massachusetts General Hospital

Chapters

00:05Title
00:57Extraction of Total Nucleic Acid from Cervicovaginal Swabs (Phenol-Chloroform Extraction)
05:52PCR Amplification of the 16S rRNA Gene V4 Hypervariable Region
09:40Library Pooling and High-Throughput Sequencing
10:30Evaluation of Nucleic Acid Extraction and 16S rRNA Gene PCR Amplification
11:35Conclusion

Summary

Automatic Translation

我们描述了从拭子用16S rRNA基因扩增子测序细菌群落特征提取核酸的高效，可靠和成本有效的方法。该方法允许对多种样品类型共同的处理方法，并容纳许多下游分析流程。

Tags

Nucleic Acid Extraction 16S RRNA Gene Sequencing Bacterial Community Characterization Swabs DNA RNA Human Samples Body Sites NaCl-Tris-EDTA Buffer SDS Phenol Chloroform Isoamyl Alcohol Bead Beading Homogenization Centrifugation

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