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Biology
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高效的核酸提取和16S rRNA基因序列的细菌群落特征
JoVE Journal
Biology
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JoVE Journal
Biology
Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Please note that all translations are automatically generated.
Click here for the English version.
高效的核酸提取和16S rRNA基因序列的细菌群落特征
DOI:
10.3791/53939-v
•
12:37 min
•
April 14, 2016
•
Melis N. Anahtar
,
Brittany A. Bowman
,
Douglas S. Kwon
1
Ragon Institute of MGH, MIT, and Harvard
,
Massachusetts General Hospital
Chapters
00:05
Title
00:57
Extraction of Total Nucleic Acid from Cervicovaginal Swabs (Phenol-Chloroform Extraction)
05:52
PCR Amplification of the 16S rRNA Gene V4 Hypervariable Region
09:40
Library Pooling and High-Throughput Sequencing
10:30
Evaluation of Nucleic Acid Extraction and 16S rRNA Gene PCR Amplification
11:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了从拭子用16S rRNA基因扩增子测序细菌群落特征提取核酸的高效,可靠和成本有效的方法。该方法允许对多种样品类型共同的处理方法,并容纳许多下游分析流程。
Tags
Nucleic Acid Extraction
16S RRNA Gene Sequencing
Bacterial Community Characterization
Swabs
DNA
RNA
Human Samples
Body Sites
NaCl-Tris-EDTA Buffer
SDS
Phenol Chloroform Isoamyl Alcohol
Bead Beading
Homogenization
Centrifugation
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