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Gelijktijdig Beoordeling van Cardiomyocyte DNA Synthese en Ploïdie: een methode om Assist Kwantificering van Cardiomyocyte Regeneration en Omzet
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
DOI:

08:03 min

May 23, 2016

Chapters

  • 00:05Title
  • 00:48Heart Harvest
  • 02:13Nuclei Dissociation and Cardiomyocyte Nuclei Labeling
  • 04:51EdU Incorporation Detection
  • 06:05Results: EdU Labeled Cardiomyocyte Quantification in WT and Mdx Hearts
  • 07:03Conclusion

Summary

Automatic Translation

Quantification of cardiomyocyte turnover is challenging. The protocol described here makes an important contribution to this challenge by enabling accurate and sensitive quantification of neo-cardiomyocyte nuclei generation and nuclei ploidy.

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