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Chapters
- 00:05Title
- 00:54Arranging and Aligning the Exitation Path
- 04:01Alignment of Polarization Rotator and Emission Path
- 06:42System Synchronization and Calibration
- 08:23Results: Multi-colored, High Speed TIRF Microscopy
- 10:11Conclusion
This article provides an in depth guide for the assembly and operation of a structured illumination microscope operating with total internal reflection fluorescence illumination (TIRF-SIM) to image dynamic biological processes with optical super-resolution in multiple colors.
