Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Generation of Marked and Markerless Mutants in…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
 

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

Article DOI: 10.3791/54001-v • 11:45 min • May 29th, 2016
May 29th, 2016 •
1, 1, 1
1Department of Biochemistry, University of Cambridge

Chapters

Summary
Automatic Translation

Introducing multiple genomic alterations into cyanobacteria is an essential tool in the development of strains for industrial and basic research purposes. We describe a system for generating unmarked mutants in the model cyanobacterial species Synechocystis sp. PCC6803 and marked mutants in Synechococcus sp. PCC7002.

Tags

Keywords: Cyanobacteria Synechocystis Synechococcus Unmarked Mutants Marked Mutants Antibiotic Resistance Negative Selectable Marker Genetic Manipulation BG11 Medium Transformation Kanamycin PCR Knockout
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter