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体外测定菌落为表征的三效祖细胞从成人胰腺小鼠隔离

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Developmental Biology

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JoVE Journal Developmental Biology

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

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体外测定菌落为表征的三效祖细胞从成人胰腺小鼠隔离

DOI:

10.3791/54016-v

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09:31 min

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June 10, 2016

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Jacob R. Tremblay*^1,2, Jeanne M. LeBon*¹, Angela Luo, Janine C. Quijano, Lena Wedeken, Kevin Jou, Arthur D. Riggs, David A. Tirrell, H. Teresa Ku

¹Diabetes and Metabolism Research Institute,Beckman Research Institute of City of Hope, ²Irell & Manella Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, ³Division of Chemistry and Chemical Engineering,California Institute of Technology

Chapters

00:05Title
01:22Adult Murine Pancreata Single-cell Suspension Generation
04:33Colony Whole Mount Immunostaining
07:39Results: Representative Analyses of Colonies Derived from PCFU (Pancreatic Colony Forming Unit)
08:39Conclusion

Summary

Automatic Translation

体外集落测定法来检测自我更新和从成年鼠胰腺分离的祖细胞的分化被设计的。在这些测定中，胰祖细胞产生细胞集落在3维空间中含有甲基纤维素半固体培养基。用于处理单个细胞和单独的菌落的特征的协议进行了描述。

Tags

In Vitro Colony Assay Tri-potent Progenitor Cells Adult Murine Pancreas Pancreatic Progenitor Cells Self-renewal Differentiation Hematopoietic Stem Cells Pancreatic Cell Populations Abdominal Cavity Spleen PBS BSA DNase I Collagenase B Cell Isolation Cell Culture

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