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Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
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JoVE Journal Medicine
Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing

DOI:
10.3791/54026-v

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09:24 min

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June 09, 2016

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, , , , , , ,
1Biomedical Engineering,University of Alabama at Birmingham, 2Radiation Oncology,University of Alabama at Birmingham, 3Neurosurgery,University of Alabama at Birmingham, 4Vivo Biosciences, Inc.

Chapters

  • 00:05Title
  • 01:31Tumor Disaggregation
  • 04:05Microtumor Generation and Drug Dosing
  • 05:54Microtumor Morphologic and Phenotypic Analyses
  • 07:08Results: Representative Microtumor Growth and Analyses
  • 08:38Conclusion

Summary

Automatic Translation

Automatic Translation

Patient-derived xenografts of glioblastoma multiforme can be miniaturized into living microtumors using 3D human biogel culture system. This in vivo-like 3D tumor assay is suitable for drug response testing and molecular profiling, including kinomic analysis.

Tags

Keywords: 3D Biogel CulturePatient-derived GlioblastomaKinomic ProfilingDrug Response TestingMicrotumor GenerationTumor DisaggregationPatient-derived Xenograft CellsCell DissociationTumor MincingEnzyme Digestion

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