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Design og Udvikling af aptamer-Gold Nanopartikel kolorimetrisk Analyser for In-the-field Applications
JoVE Journal
Biology
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JoVE Journal Biology
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
DOI:

08:23 min

June 23, 2016

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Chapters

  • 00:05Title
  • 00:47Synthesis via Citrate Reduction of Gold Nanoparticles (AuNP) and Characterization
  • 02:35DNA-aptamer, Buffer, Solution, and Assay Preparation
  • 03:35Salt Titration and Assay Setup
  • 05:21Freezing Aptamer–AuNP Assay for Long-term Storage
  • 06:38Results: Assay Response with Changing DNA Coverage Density and Performance of the Assay for Long-term Storage
  • 07:37Conclusion

Summary

Automatic Translation

Designet og udviklingen af ​​en aptamer-guld nanopartikel kolorimetrisk assay til påvisning af små molekyler for in-the-field ansøgninger blev undersøgt. Endvidere blev en smart-enhed kolorimetrisk applikation (app) valideret og langtidsopbevaring af assayet blev etableret til anvendelse i marken.

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