Journal
/
Medicine
/
Påvisning af Anastasis In Vivo af CaspaseTracker Biosensor
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Påvisning af Anastasis In Vivo af CaspaseTracker Biosensor

DOI:
10.3791/54107-v

•

20:16 min

•

February 01, 2018

•

, ,
1Institute for Basic Biomedical Sciences,Johns Hopkins University School of Medicine, 2School of Life Sciences,Chinese University of Hong Kong, 3Department of Neurosurgery,Johns Hopkins University School of Medicine

Chapters

  • 00:05Title
  • 04:50Preparing the Caspase Tracker Biosensor Flies
  • 09:29Transient Cell Death Induction
  • 11:10Fixation and Staining of Egg Chambers
  • 13:58Results: Reversal of Cell Death Process in Drosophila
  • 18:44Conclusion

Summary

Automatic Translation

Automatic Translation

Anastasis teknisk udfordrende for at opdage i vivo , fordi de celler, der har vendt den celle død proces kan være morfologisk umulig at skelne fra normale sunde celler. Her beskriver vi protokoller til påvisning og spore celler, der undergår anastasis i levende dyr ved hjælp af vores nyudviklede i vivo CaspaseTracker biosensor system.

Tags

AnastasisCaspaseApoptosisCell DeathCaspaseTracker BiosensorProgrammed Cell DeathRtTACre LoxPDsRedLive Cell Microscopy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54107?article_type=v

In Vivo Canine Muscle Function Assay
check_url/54107?article_type=v

In vivo Dual Substrate Bioluminescent Imaging
check_url/54107?article_type=v

A Research Method For Detecting Transient Myocardial Ischemia In Patients With Suspected Acute Coronary Syndrome Using Continuous ST-segment Analysis
check_url/54107?article_type=v

In vivo 19F MR for Cell Tracking
check_url/54107?article_type=v

Afsløring Abnormiteter i Choroidal karrene i en musemodel af Aldersrelateret makuladegeneration af Time-kursus indocyaningrønt angiografi
check_url/54107?article_type=v

Intrauterin Telemetri måler Mouse kontraktile Pressure<em> In Vivo</em
check_url/54107?article_type=v

Rose Bengal Photothrombosis ved Konfokal Optical Imaging<em> In vivo</em>: En model af Single Vessel Stroke
check_url/54107?article_type=v

Udløsning Reactive gliose<em> In vivo</em> Af en forhjerne Stab Skade
check_url/54107?article_type=v

Generation af Microtumors Brug 3D Menneskelig Biogel Kultur System og Patient-afledte glioblastomaceller for Kinomic Profilering and Drug respons Testing
check_url/54107?article_type=v

Udtømning af Mouse Celler fra Humane tumorxenoplantater Markant Forbedrer Downstream Analyse af Target Cells

Read Article