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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
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Biochemistry
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JoVE Journal Biochemistry
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

DOI:
10.3791/54144-v

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08:27 min

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October 01, 2016

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, , , ,
1Synthetic Biology & Bioengineering Research Center,Korea Research Institute of Bioscience and Biotechnology, 2College of Interdisciplinary & Creative Studies,Konyang University, 3Biosystems and Bioengineering Program,University of Science and Technology

Chapters

  • 00:05Title
  • 00:48Förster Resonance Energy Transfer (FRET) Biosensor Preparation
  • 04:25Analysis of Sugar Content of Beverages Using a FRET Biosensor
  • 06:31Results: Quantitative Analysis of Sugar Content Using a Protein Biosensor for Portable FRET Analyzer
  • 07:49Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.

Tags

Keywords: Fluorescence DetectionFRET AnalyzerSugar ContentSmall Molecule SensingBiosensorE. ColiPlasmidElectroporationLB AgarSeed CultureIPTG

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