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Teknikker for utviklingen av Robust Pentose-fermen gjær for biokonversjon av lignocellulose til Etanol
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Chapters
- 00:05Title
- 01:40Enrichment of Robust Derivatives during Serial Transfer on AFEX CSH
- 03:42Isolation of Single Cell Tolerant Derivatives after Enrichment on AFEX CSH
- 04:58Performance Evaluation of AFEX CSH Tolerant Derivatives Compared to Parent
- 07:15Applying Continuous Culture to Select for Ethanol-challenged Xylose Utilization
- 09:43Isolation of Single-cell Colonies That Utilize Xylose in PSGHL When Ethanol Is Present
- 11:20Results: Derivatives of S. stipitis Parent Strain Have Improved Efficiency in Converting Hydrolyzates to Ethanol
- 12:54Conclusion
Adaptive evolusjon og isoleringsteknikker er beskrevet og demonstrert å gi derivater av Scheffersomyces stipitis stamme NRRL Y-7124, som er i stand til hurtig å konsumere heksose- og pentose- blandede sukkerarter i enzym-saccharified undetoxified hydrolysater og for å akkumulere over 40 g / l etanol.
