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Impression Cytology of the Lid Wiper Area
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JoVE Journal Medicine
Impression Cytology of the Lid Wiper Area

Impression Cytology of the Lid Wiper Area

DOI:
10.3791/54261-v

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07:01 min

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August 09, 2016

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, , ,
1Centre for Contact Lens Research,University of Waterloo

Chapters

  • 00:05Title
  • 00:44Cell Collection
  • 01:56Sample Processing
  • 03:48Sample Imaging
  • 05:14Results: Representative Lid Wiper Cell Imaging
  • 06:12Conclusion

Summary

Automatic Translation

Automatic Translation

We collected, stained and imaged cells from the conjunctiva of the inner eyelid margin of human subjects. By characterizing cell morphology and metabolic activity, this method may further our understanding of dry eye and the role that friction between the ocular surfaces may play in perceiving ocular discomfort.

Tags

Keywords: Impression CytologyLid Wiper AreaOcular SurfaceDry EyeContact Lens DiscomfortEpithelial CellsTopical AnestheticPBSOcular LubricantsMembraneEthanolImmunocytochemical StainingCytological Staining

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