JoVE Journal
Developmental Biology
Developmental Biology
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately.